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KMID : 0545119990090010044
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Journal of Microbiology and Biotechnology
1999 Volume.9 No. 1 p.44 ~ p.49
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Cellulase Production in Fed-Batch Culture by Trichoderma reesei Rut C30
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Yu, Xiao Bin
Yun, Hyun Shik/Koo, Yoon Mo
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Abstract
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Cellulase production by fed-batch cultivation of Trichoderma reesei Rut C30 with various initial concentrations of Solka Floc in 1％ wheat bran-containing medium was investigated. The cellulase activity and productivity increased with initial Solka Floc concentration up to 5％. When a total Solka Floc concentration of 90 g/l was used for cellulase production, CMC (carboxymethyl cellulose) and FP (filter paper) activities. productivity, and yield were 359.7 U/㎖, 30.61 U/㎖, 161 FPU L^1 h^i. and 340 FPU g , respectively. It was important to maintain a high cell concentration during cellulase production to obtain high cellulase activity and productivity. Cellulase powder was prepared by ammonium sulfate precipitation: FP activity was 396.7 U/g and CMC activity was 6481 U/g.
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